A fluoride ion deprotection strategy in peptide synthesis. Combination with selective deprotection using the dilute methanesulfonic acid of .ALPHA.-amino protecting groups.
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چکیده
منابع مشابه
Acid cleavage/deprotection in Fmoc/tBu solid-phase peptide synthesis.
In general, a solid-phase peptide synthesis (SPPS) consists of the assembly of a protected peptide chain on a polymeric support (=synthetic step) and the subsequent cleavage/deprotection to release the crude, deprotected peptide from the solid support (=cleavage step). Usually, these two steps are followed by chromatographic purification of the crude peptide (see Chapters 1,2,4, and 5, PAP). Th...
متن کاملFluoride ion promoted deprotection and transesterification in nucleotide triesters.
Tetrabutylammonium fluoride will remove phenyl, trichloroethyl and cyanoethyl groups from nucleotides. In addition to the desired nucleotide products other results including chain cleavage, phosphofluoridates and cyanoethylated thymidine units may be obtained depending on the conditions used. Fluoride ion has been used to successfully exchange phenyl and trichloroethyl groups for methyl, ethyl ...
متن کاملPurification and use of E. coli peptide deformylase for peptide deprotection in chemoenzymatic peptide synthesis.
Peptide deformylases (PDFs) catalyze the removal of the formyl group from the N-terminal methionine residue in nascent polypeptide chains in prokaryotes. Its deformylation activity makes PDF an attractive candidate for the biocatalytic deprotection of formylated peptides that are used in chemoenzymatic peptide synthesis. For this application it is essential to use PDF preparations that are free...
متن کاملAmino acid-protecting groups.
Albert Isidro-Llobet, Mercedes Álvarez, Fernando Albericio Institute for Research in Biomedicine, Barcelona Science Park, Baldiri Reixac 10, 08028Barcelona, Spain. CIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Barcelona Science Park, Baldiri Reixac 10, 08028-Barcelona, Spain. Laboratory of Organic Chemistry, Faculty of Pharmacy, University of Barcelona, 08028Bar...
متن کاملMild, selective deprotection of PMB ethers with triflic acid/1,3-dimethoxybenzene
An efficient method for the cleavage of the p-methoxybenzyl protecting group of several alcohols in the presence of 0.5 equiv of trifluoromethanesulfonic acid and 1,3-dimethoxybenzene in dichloromethane at room temperature is described. 2011 Elsevier Ltd. All rights reserved. O OAc Me HO PMBO O-allyl 1 O AcO AcO NPhth AcO O NH CCl3 2 O OAc Me O O AcO AcO AcO
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1988
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.36.5024